Journal: Acta biochimica et biophysica Sinica

Article Title: TXNIP aggravates cardiac fibrosis and dysfunction after myocardial infarction in mice by enhancing the TGFB1/Smad3 pathway and promoting NLRP3 inflammasome activation.

doi: 10.3724/abbs.2023150

Figure Lengend Snippet: Figure 3. TXNIP promoted post-MI collagen deposition (A,B) Representative picrosirius red staining images of heart sections from each group at 28 days after treatment (A) under a light microscope and (B) under a polarized light microscope. Collagen I showed red/orange birefringence, while Collagen III showed green/whitish birefringence. (C,E,G) Representative immunohistochemical staining images of heart sections from each group at 28 days after treatment. (C) Collagen I deposition. (E) Collagen III deposition, and (G) ACTA2 deposition. (D) Statistical analysis of Collagen I deposition. Data are shown as the mean±SEM, n=3. **P<0.01. (F) Statistical analysis of Collagen III deposition. Data are shown as the mean± SEM, n=3. **P<0.01. (H) Statistical analysis of ACTA2 deposition. Data are shown as the mean±SEM, n=3. *P<0.05, **P<0.01.

Article Snippet: Then, the membrane was incubated with one of the following antibodies at 4°C overnight: rabbit antiCollagen I, ACTA2, CTGF, TXNIP, Smad3, p-Smad3 (Abcam), antiSmad7, NLRP3, ASC, Caspase-1/Cleaved Caspase-1, Mature IL1B, GAPDH (Wanleibio, Shenyang, China), mouse anti-TGFB1 antibody (Santa Cruz Biotechnology, Dallas, USA), and mouse anti-Collagen III antibody (Boster).

Techniques: Staining, Light Microscopy, Immunohistochemical staining

Journal: Acta biochimica et biophysica Sinica

Article Title: TXNIP aggravates cardiac fibrosis and dysfunction after myocardial infarction in mice by enhancing the TGFB1/Smad3 pathway and promoting NLRP3 inflammasome activation.

doi: 10.3724/abbs.2023150

Figure Lengend Snippet: Figure 4. TXNIP increased post-MI collagen deposition (A) Representative images of the protein levels of Collagen III, Collagen I, ACTA2, and CTGF. (B‒E) Statistical analyses of the protein levels of Collagen I, Collagen III, ACTA2, and CTGF. Data are shown as the mean±SEM, n=3‒5. *P<0.05, **P<0.01; ns, not significant.

Article Snippet: Then, the membrane was incubated with one of the following antibodies at 4°C overnight: rabbit antiCollagen I, ACTA2, CTGF, TXNIP, Smad3, p-Smad3 (Abcam), antiSmad7, NLRP3, ASC, Caspase-1/Cleaved Caspase-1, Mature IL1B, GAPDH (Wanleibio, Shenyang, China), mouse anti-TGFB1 antibody (Santa Cruz Biotechnology, Dallas, USA), and mouse anti-Collagen III antibody (Boster).

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bazi Bushen alleviates skin senescence by orchestrating skin homeostasis in SAMP6 mice

doi: 10.1111/jcmm.17833

Figure Lengend Snippet: Effects of Bazi Bushen on epidermal stem cells in SAMP6 mice. (A) Western blot analysis of OCT4, SOX2, NANOG and GAPDH in skin tissues. (B) Quantification of band intensities of OCT4, SOX2 and NANOG relative to GAPDH in skin tissues. (C) Representative immunofluorescence images of COL17A1+ cells in skin tissues. (D) Western blot analysis of COL17A1 and GAPDH in skin tissues. (E) Quantification of band intensities of COL17A1 relative to GAPDH in skin tissues. Data are expressed as mean ± SD. ** p < 0.01 and * p < 0.05 versus the SAMP6 group ( n = 3).

Article Snippet: Mouse Matrix metalloproteinase 2 (MMP2, No. JYM0019Mo), Mouse Matrix metalloproteinase 9 (MMP9, No. JYM0737Mo) and Mouse Vascular endothelial growth factor (VEGF, No. JYM0258Mo) ELISA kits were purchased from Jiyinmei Biological Technology Co., Ltd. Rabbit integrin subunit alpha 6 (ITGA6) Monoclonal Antibody BM4348 and Rabbit hemidesmosome component collagen XVII (COL17A1) Monoclonal Antibody BM4360 were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Western Blot, Immunofluorescence